1. General work procedures


Laboratory rules:

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  • Everyone is responsible for maintaining a clean and organized work area, use bench paper!
  • Your work area should be available for others to use when you are not present.
  • The work area and equipment should be cleaned after use.
  • Equipment brought from other areas should be returned, in a clean condition.
  • Spills on benches, balances, etc., should be wiped away immediately with absorbent paper.
  • Food and drink is strictly prohibited in the laboratory.
  • Appropriate protective clothing, gloves, safety glasses etc. should be worn when required.


Disinfection methods

1. Chemical disinfection

  • 70 % ethanol – disinfection of skin and equipment when spill involves bacteria or virus.
  • Chlorhexidine or Pyricept – disinfection of skin and cuts/wounds.
  • Hypochlorite ( ”chlorine”) – disinfection of equipment.
  • Virkon – for disinfection of non-autoclavable equipment used for bacteria and viruses.


2. Autoclaving

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  • Autoclave is used to sterilize equipment and solutions.
  • The autoclave should not be used without training. Follow the program for each autoclave.
  • Autoclaves are found in these rooms:

Follow the instructions for each individual autoclave.



3. UV-light

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  • UV-light is used for disinfection of i.e. work benches as well as for destroying DNA/RNA.
  • The UV-light must not be on when working!
  • 10 minutes irradiation with UV-light on a flat surface should normally be sufficient to kill all microorganisms. If the UV-light has been on overnight, it is recommended to not use the room for approximately 30 minutes (in order to remove ozone) after the UV light has been switched off.

Consumables and test tubes

  • Infected glassware and solutions/media should be sterilized with autoclaving.
  • Everyone is responsible for washing and replacing glassware according to the rules  in the lab where work is conducted. The same goes for equipment and solutions which are to be autoclaved or disinfected in another way.
  • NB: Refill shared buffers, test tubes, sterile glassware, pipettes, etc. when empty so that the equipment is ready for the next user.